1. INRODUCTION TO MICROSCOPY

1.2. HOW TO OPERATE A MICROSCOPE

  • Start with the lowest power: 
    Rotate the nosepiece to click the lowest power objective lens into position. 
  • Place the slide: 
    Secure the slide on the stage, making sure the specimen is over the hole in the stage. 
  • Position the objective lens: 
    Use the coarse focus knob to move the stage up until the objective lens is very close to the slide, but not touching it. 
  • Look through the eyepiece: 
    Slowly turn the coarse focus knob to move the stage away from the lens until the specimen comes into view. 
  • Sharpen the focus: 
    Use the fine adjustment knob to get a clear, sharp image. 
  • Adjust the light: 
    Use the diaphragm to control the amount of light. You may need to increase the light for higher magnifications.